Construction of dicentric and translocation dose response curves by the FISH assay. Evaluation of ionizing radiation exposure for biodosimetry: Validation of Whole Chromosome

Strong Correlation among Three Biodosimetry Techniques Following Exposures to Ionizing Radiation

Three in vitro dose calibration curves for biodosimetry such as dicentric chromosome assay, fluorescence in situ hybridization (FISH) assay for translocation, and micronuclei (MNs) in binucleated cell assay were established after exposure to ionizing radiation. Peripheral blood lymphocyte samples obtained from healthy donors were irradiated with 60Co source at a dose rate of 0.5 Gy/min to doses...

Biological Dose Assessment by Chemical Induction of Premature Chromosome Condensation

Dose assessment of radiation victims is a key element in medical management of radiation accidents. At high radiation doses (> 6 Gy), dicentric assay lose its efficiency and premature chromosome condensation (PCC) assay was proposed to overcome the restrictions of dicentric assay. It was shown that PCC ring is more suitable and simpler than dicentric for biodosimetry in high radiation doses. In...

Validation of in vivo translocation frequency obtained by FISH with conventional G-banding Evaluation of ionizing radiation exposure for biodosimetry: Validation of Whole Chromosome

مروری بر انواع روش‌های سیتوژنتیک در دزیمتری بیولوژیکی

The growing use of ionizing radiation in various sectors of industry, medicine, agriculture and research has increased the risk of radiation exposure of staff and people. Ionizing radiation as a strong clastogen can cause different types of DNA damages and chromosomal aberrations. Biological dosimetry, based on the cytogenetic analysis of peripheral blood lymphocytes has a wide application in d...

Retrospective Biodosimetry of an Occupational Overexposure-Case Study.

In 2014, Health Canada was approached by the Canadian Nuclear Safety Commission to conduct biodosimetry for a possible overexposure 4 y prior to assessment. Dose estimates were determined by means of two cytogenetic assays, the dicentric chromosome assay (DCA) and translocations as measured by the fluorescent in situ hybridization (FISH). As dicentrics are considered to be unstable over time, t...